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KMID : 0371319960500020149
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Journal of the Korean Surgical Society
1996 Volume.50 No. 2 p.149 ~ p.155
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Evaluation of Graft-Survival, a Study Using Auxiliary Partial Liver Transplantation in the Rat
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유창현
김준현/윤선회/김병호
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Abstract
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In experimental transplantation, auxiliary partal liver transplantation (APLT) which has several technical adventages is used conveniently in the rat The partial liver graft can survive with only a portal blood supply in the rat, but a large
portion of
the transplants fail because of complications with microvasculature and in biliary reconstruction. Some of the surviving animals may have failed grafts whose host livers were supplied by the hepatic artery and portal revascularization of the
host-liver.
We evaluated the true graft survival in the survived experimental animals by means of radiologic splenoportogram(SPG), whose findings divided the experimental animals into three groups: visualization of well regenerated grafts as group 1,
moderately
regenerated grafts as group 2 and visualization of nonatrophied host liver as group 3.
All the experimental animals were underwent an exploratory laparotomy t observe the gross findings of the grafts and take biopsy specimens. We graded the gross findings of grafts for each animal, on a scale of grades 1-3 corresponding to god,
fair,
or
bad color and degree of regeneration. The microscopic findings were graded on a scale of grades 1-3, corresponding to mild, moderate, or severe bile duct proliferation, inflammatory cell infiltration, and destruction of lobular architecture. A
successful transplant was finally confirmed by long-term survival after host-hepatectomy at 3 weeks post-transplant.
As results, group 1; n=14 group 2; n=10 and group 3; n=6 were classified by the findings of a portogram. All the animals of group 1 presented grade 1 gross and microscopic findings and survived more than 3 months after host-hepatectomy at 3 weeks
after
transplantation. On the contrary, almost cases of group 2 and group 3 failed long-term survival after host-hepatectomy except one animal that showed grade 2 findings. The results revealed that findings of a well-regenerated graft and
nonvisualization of
host liver by a portogram were implicated in the true graft-survival among the survived animals.
In this study we suggest that the simple SPG can exactly predict the fate of a graft which increases the reliability of the study using APLT in the rat.
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